Journal: Bioactive Materials

Article Title: Activatable companion theranostics for dual-modality imaging-escorted pyroptosis-propelled synergistic cancer therapy

doi: 10.1016/j.bioactmat.2025.08.036

Figure Lengend Snippet: In vitro mechanism of synergistic therapy mediated by LET-Cl@GOx. Changes of a extracellular acidification rate (ECAR) and b relevant glycolysis ability after treatments. Variations of c mitochondrial oxygen consumption rate (OCR) and d their associated quantifications. e Measurements of ATP production rate with various treatments. f Brightfield images of A549 cells following different treatments. Scale bar: 50 μm g FL images of cells stained with Annexin V-FITC/PI after various treatments. Scale bar: 50 μm h Immunofluorescence staining images of caspase-3 activation. Scale bar: 50 μm. i Western blotting analysis of indicated protein expression following treatments. j Measurements of LDH release after indicated treatments. k The process of pyroptosis through the Caspase-3/GSDME pathway induced by LET-Cl@GOx (created with BioRender.com ). The data are presented as the means ± SDs, n = 3. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. The symbols “+” indicate laser irradiation (808 nm, 0.6 W cm −2 ).

Article Snippet: Afterwards, the cells were stained Mitochondrial Membrane Potential Assay Kit (Beyotime, China).

Techniques: In Vitro, Staining, Immunofluorescence, Activation Assay, Western Blot, Expressing, Irradiation

Journal: PLoS ONE

Article Title: Berberine Induces Caspase-Independent Cell Death in Colon Tumor Cells through Activation of Apoptosis-Inducing Factor

doi: 10.1371/journal.pone.0036418

Figure Lengend Snippet: Cells were treated with berberine at indicated concentrations for 18 h (A and B), as described in . Intracellular ROS levels were detected using DHE probe and the amount of fluorescence was measured using flow cytometry. The fold change of the amount of fluorescence was calculated by comparing that in the treated groups to the control group (A). Cells were treated with the JC-1 probe and then analyzed by flow cytometry to measures the fraction of cells with polarized or depolarized mitochondrial membrane (B). Cells were treated with berberine at 50 µM for 18 h in the presence or absence of 5 µM of Tiron, a ROS scavenger. Cell number change (C) and LDH release (D) were detected as described in . * p <0.01 compared to the control group, # p <0.01 compared to the berberine treated group.

Article Snippet: Δ ψ was measured using the Flow Cytometry Mitochondrial Membrane Potential Detection kit (BD™ Mitoscreen) according to the manufacturer’s instructions.

Techniques: Fluorescence, Flow Cytometry